Enzyme Experiments :: Free Essays

Enzyme Experiments Central Experiment The activity Of An Enzyme, e.g., amylase converting stiffen to reducing saccharide. 1. Substrate starch - uptake the very(prenominal) vividness of the same starch settlement separately time 2. Enzyme amylase - use the same volume of the same amylase solution each time 3. Only use starch and amylase solutions that screen negative for reducing sugar with Benedicts Reagent 4. Four test tubes individually one third full of (a) starch solution, (b) amylase solution, (c) starch solution and (d) water 5. Warm all to 37C in a heated water bath at 37C 6. motley (a) and (b) - the experiment. Mix (c) and (d) - the control 7. Use a buffer to maintain the pH at a constant suitable value pH 8 8. Every minute test a small sample of each for starch using iodine 9. Control results always blue-black - starch always present, starch not low-toned down 10. Experiment Result e ventually a yellow-brown colour - starch is not present, it has been broken down 11. Now test the experiment solution for reducing sugar using Benedicts Reagent. Result brick-red colour formed - reducing sugar is forthwith present 12. Repeat the entire procedure many times to cast the results 13. Conclusion amylase converts starch to reducing sugar. Only when amylase is present does starch run and reducing sugar appear Note Fehlings can be use to test for reducing sugar. Experiment To Determine the Effect of Enzyme Concentration on the Rate of Enzyme Action. Same as the central experiment, at different amylase concentrations, as far as 10. Make up different dilutions of the amylase solution by adding increasing amounts of water.